During the epithelial-to-mesenchymal transition, the intracellular cytoskeleton undergoes severe reorganization which allows epithelial cells to transition into a motile mesenchymal phenotype. Among the different cytoskeletal elements, the intermediate filaments keratin (in epithelial cells) and vimentin (in mesenchymal cells) have been demonstrated to be useful and reliable histological markers. In this study, we assess the potential invasiveness of six human breast carcinoma cell lines and two mouse fibroblasts cells lines through single cell migration assays in confinement. We find that the keratin and vimentin networks behave mechanically…(mehr)
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%0 Journal Article
%1 ficorella2021intermediate
%A Ficorella, Carlotta
%A Eichholz, Hannah Marie
%A Sala, Federico
%A Vázquez, Rebeca Martínez
%A Osellame, Roberto
%A Käs, Josef A
%D 2021
%I IOP Publishing
%J New Journal of Physics
%K zno
%N 8
%P 083028
%T Intermediate filaments ensure resiliency of single carcinoma cells, while active contractility of the actin cortex determines their invasive potential
%V 23
%X During the epithelial-to-mesenchymal transition, the intracellular cytoskeleton undergoes severe reorganization which allows epithelial cells to transition into a motile mesenchymal phenotype. Among the different cytoskeletal elements, the intermediate filaments keratin (in epithelial cells) and vimentin (in mesenchymal cells) have been demonstrated to be useful and reliable histological markers. In this study, we assess the potential invasiveness of six human breast carcinoma cell lines and two mouse fibroblasts cells lines through single cell migration assays in confinement. We find that the keratin and vimentin networks behave mechanically the same when cells crawl through narrow channels and that vimentin protein expression does not strongly correlate to single cells invasiveness. Instead, we find that what determines successful migration through confining spaces is the ability of cells to mechanically switch …
@article{ficorella2021intermediate,
abstract = {During the epithelial-to-mesenchymal transition, the intracellular cytoskeleton undergoes severe reorganization which allows epithelial cells to transition into a motile mesenchymal phenotype. Among the different cytoskeletal elements, the intermediate filaments keratin (in epithelial cells) and vimentin (in mesenchymal cells) have been demonstrated to be useful and reliable histological markers. In this study, we assess the potential invasiveness of six human breast carcinoma cell lines and two mouse fibroblasts cells lines through single cell migration assays in confinement. We find that the keratin and vimentin networks behave mechanically the same when cells crawl through narrow channels and that vimentin protein expression does not strongly correlate to single cells invasiveness. Instead, we find that what determines successful migration through confining spaces is the ability of cells to mechanically switch …},
added-at = {2025-02-20T11:45:20.000+0100},
author = {Ficorella, Carlotta and Eichholz, Hannah Marie and Sala, Federico and Vázquez, Rebeca Martínez and Osellame, Roberto and Käs, Josef A},
biburl = {https://puma.scadsai.uni-leipzig.de/bibtex/223ebdaf259e568ad019376a5da854139/scadsfct},
citation = {New Journal of Physics 23 (8), 083028, 2021},
interhash = {58611026d7504108207c8b4b8abf0e24},
intrahash = {23ebdaf259e568ad019376a5da854139},
journal = {New Journal of Physics},
keywords = {zno},
number = 8,
pages = 083028,
publisher = {IOP Publishing},
timestamp = {2025-02-20T11:45:20.000+0100},
title = {Intermediate filaments ensure resiliency of single carcinoma cells, while active contractility of the actin cortex determines their invasive potential},
volume = 23,
year = 2021
}
