The fetal membranes are essential for the maintenance of pregnancy, and their integrity until parturition is critical for both fetal and maternal health. Preterm premature rupture of the membranes (pPROM) is known to be an indicator of preterm birth, but the underlying architectural and mechanical changes that lead to fetal membrane failure are not yet fully understood. The aim of this study was to gain new insights into the anatomy of the fetal membrane and to establish a tissue processing and staining protocol suitable for future prospective cohort studies.In this proof of principle study, we collected fetal membranes from women undergoing vaginal delivery or cesarean section. Small membrane sections were then fixed, stained for nucleic acids, actin, and collagen using fluorescent probes, and subsequently imaged in three dimensions using a spinning disk confocal microscope.Four fetal …
%0 Journal Article
%1 eichholz2024anatomy
%A Eichholz, Hannah Marie
%A Cornelis, Alissa
%A Wolf, Benjamin
%A Grubitzsch, Hanna
%A Friedrich, Philip
%A Makky, Ahmad
%A Aktas, Bahriye
%A Käs, Josef Alfons
%A Stepan, Holger
%D 2024
%I Springer Berlin Heidelberg
%J Archives of Gynecology and Obstetrics
%K imported topic_earthenvironment
%N 5
%P 1919-1923
%T Anatomy of the fetal membranes: Insights from spinning disk confocal microscopy
%V 309
%X The fetal membranes are essential for the maintenance of pregnancy, and their integrity until parturition is critical for both fetal and maternal health. Preterm premature rupture of the membranes (pPROM) is known to be an indicator of preterm birth, but the underlying architectural and mechanical changes that lead to fetal membrane failure are not yet fully understood. The aim of this study was to gain new insights into the anatomy of the fetal membrane and to establish a tissue processing and staining protocol suitable for future prospective cohort studies.In this proof of principle study, we collected fetal membranes from women undergoing vaginal delivery or cesarean section. Small membrane sections were then fixed, stained for nucleic acids, actin, and collagen using fluorescent probes, and subsequently imaged in three dimensions using a spinning disk confocal microscope.Four fetal …
@article{eichholz2024anatomy,
abstract = {The fetal membranes are essential for the maintenance of pregnancy, and their integrity until parturition is critical for both fetal and maternal health. Preterm premature rupture of the membranes (pPROM) is known to be an indicator of preterm birth, but the underlying architectural and mechanical changes that lead to fetal membrane failure are not yet fully understood. The aim of this study was to gain new insights into the anatomy of the fetal membrane and to establish a tissue processing and staining protocol suitable for future prospective cohort studies.In this proof of principle study, we collected fetal membranes from women undergoing vaginal delivery or cesarean section. Small membrane sections were then fixed, stained for nucleic acids, actin, and collagen using fluorescent probes, and subsequently imaged in three dimensions using a spinning disk confocal microscope.Four fetal …},
added-at = {2024-11-29T11:55:41.000+0100},
author = {Eichholz, Hannah Marie and Cornelis, Alissa and Wolf, Benjamin and Grubitzsch, Hanna and Friedrich, Philip and Makky, Ahmad and Aktas, Bahriye and Käs, Josef Alfons and Stepan, Holger},
biburl = {https://puma.scadsai.uni-leipzig.de/bibtex/2911bb0860b4bf5345647c7506f648888/joum576e},
citation = {Archives of Gynecology and Obstetrics 309 (5), 1919-1923, 2024},
interhash = {ffa32c14003d1d2a02ba513c17e18a00},
intrahash = {911bb0860b4bf5345647c7506f648888},
journal = {Archives of Gynecology and Obstetrics},
keywords = {imported topic_earthenvironment},
number = 5,
pages = {1919-1923},
publisher = {Springer Berlin Heidelberg},
timestamp = {2024-11-29T11:55:41.000+0100},
title = {Anatomy of the fetal membranes: Insights from spinning disk confocal microscopy},
volume = 309,
year = 2024
}
